Vigorous fermenters such as Escherichia coli and Entrobacter cloacae will ferment all the available sugars and then begin using the amino acids. Bacteria of the genus Micrococcus require oxygen to carry out various processes. Prepare nitrate broth in test tube and inoculate the test organism in this under aseptic conditions. When looking at the genetic tests, most of the identified strains in the Korona test are Micrococcus luteus. Lactobacillus lactis to the starter culture used in the manufacture of nitrate- ... in lactose metabolism, reduction in serum ... of wheat sourdough bread using Lactobacillus luteus and . Based on the place of origin of the bacterium that I isolated, I hypothesize that it will either be one of the above bacterium or a similar water loving organism, likely one that also forms a biofilm. The Micrococcus genus is known to be found on dust particles, in water, on skin and skin glands in vertebrates, and some species can be found in milk. The easiest way to differentiate them is by color. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, ... To confirm it is not Staphylococcus aureus, a bacitracin susceptibility test can be performed. Królasik J, Zakowska Z, Krȩpska M, Klimek L. 2010. Tille P.M (2014). The antibiotic resistance test showed only minor resistance to the antibiotic Oxacillin, which is likely due to a chance inheritance in the population or complete chance because of the weak strength. The current taxonomy of the genera of aerobic and facultatively anaerobic cocci based on genotypic and phenotypic characterization is reviewed. Another test I did to identify my isolate was DNA analysis, described in lab handouts 5 and 7. Therefore I used this as my location to take my environmental sample from. I used Prokka Genome Annotation to identify which genes are present in the bacterium, Kraken Metagenomics to identify what the DNA reads correlated to on different taxonomic levels, and SPAdes Genome Assembler to determine how many contigs were produced from the reads that were produced. Wikipedia also says that Micrococcus luteus is an obligate aerobe, backing up what my results show (2019). SIM medium should be read after an incubation of only 24 hours because a longer incubation time can cause a false negative. These results might not be trustworthy. This would be read K/A,G. Micrococcus luteus. You could also do it yourself at any point in time. Figure 3. Incuber … Explanation: Micrococcus luteus Bioceramic-based root canal sealers are considered to be an advantageous technology in endodontics. Many of the tests did line up with M. luteus though, such as the fluid thyoglycate test, which showed that it was an obligate aerobe. The colonies are a pale, translucent yellow, and are shiny when looked at in the light. When looking back at all the data, it is fairly likely that the isolate is Micrococcus luteus, especially when looking at the fluid thyoglycate test, the colony morphology, and the antibiotic susceptibility. Micrococcus varians Micrococcus luteus Staphylococcus saprophyticus Staphylococcus epidermidis ... do a lecithinase test) P. putida (Lecithinase Neg.) The outcome of laboratory and clinical studies on the … Table 2: Graph of the genomic reads by taxonomic level using Korona. Nitrate Test (Positive) ... Then a Nitrate test was performed which had a positive reaction meaning the reduction of Nitrate to Nitrite. On top of that, most of the bacterium that were stained were gram negative, which conflicts with this result. Mosby, Inc., an affiliate of Elsevier Inc. 3251 Riverport Lane, St. Louis, Missouri 63043 ; Faller A, Schleifer KH. 7.2.1.3 Copper Additionally, antibiotic susceptibility was tested using the disk diffusion test (Leigh, 2017d). • *nitrate reduction test • *oxidase test (cytochrome C oxidase) • *catalase test * Perform biochemical tests of unknown as part of Exercises 13 to 17. The nitrate reduction test detects reduced forms of nitrate, which occurs when bacteria use nitrate (NO 3) as a substitute for oxygen (O 2) during respiration. Nitrate reduction test. What species of Micrococcus gives a positive nitrate reduction test? The API 20 E test strip results revealed nothing about what the isolate uses as an electron acceptor because every result was negative, and that it likely only uses glucose as an energy and carbon source and oxygen as its electron acceptor. Un obligatoire aérobie, M. luteus se trouve danssol,poussière,eau etair, ettant que partie de la flore normale de la peaumammifère. They are generally strict aerobes and can generally reduce nitrate. The confidence on that reading is decently confident, evidenced by the blue coloring. I isolated the DNA by lysing the cell and using the Power Sol DNA Isolation Kit to isolate the DNA from the other parts of the cell. Then to actually isolate a bacterium, I chose a colony from the initial plate and did a quadrant streak of it to further isolate the bacterium, and then incubated it at 38 degrees Celsius for a week.
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